Nature Communications (Jun 2020)
An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism
- Mitchell G. Thompson,
- Jacquelyn M. Blake-Hedges,
- Jose Henrique Pereira,
- John A. Hangasky,
- Michael S. Belcher,
- William M. Moore,
- Jesus F. Barajas,
- Pablo Cruz-Morales,
- Lorenzo J. Washington,
- Robert W. Haushalter,
- Christopher B. Eiben,
- Yuzhong Liu,
- Will Skyrud,
- Veronica T. Benites,
- Tyler P. Barnum,
- Edward E. K. Baidoo,
- Henrik V. Scheller,
- Michael A. Marletta,
- Patrick M. Shih,
- Paul D. Adams,
- Jay D. Keasling
Affiliations
- Mitchell G. Thompson
- Joint BioEnergy Institute
- Jacquelyn M. Blake-Hedges
- Joint BioEnergy Institute
- Jose Henrique Pereira
- Joint BioEnergy Institute
- John A. Hangasky
- Department of Chemistry, University of California-Berkeley
- Michael S. Belcher
- Joint BioEnergy Institute
- William M. Moore
- Joint BioEnergy Institute
- Jesus F. Barajas
- Joint BioEnergy Institute
- Pablo Cruz-Morales
- Joint BioEnergy Institute
- Lorenzo J. Washington
- Joint BioEnergy Institute
- Robert W. Haushalter
- Joint BioEnergy Institute
- Christopher B. Eiben
- Joint BioEnergy Institute
- Yuzhong Liu
- Joint BioEnergy Institute
- Will Skyrud
- Department of Chemistry, University of California-Berkeley
- Veronica T. Benites
- Joint BioEnergy Institute
- Tyler P. Barnum
- Department of Plant and Microbial Biology, University of California-Berkeley
- Edward E. K. Baidoo
- Joint BioEnergy Institute
- Henrik V. Scheller
- Joint BioEnergy Institute
- Michael A. Marletta
- Department of Chemistry, University of California-Berkeley
- Patrick M. Shih
- Joint BioEnergy Institute
- Paul D. Adams
- Joint BioEnergy Institute
- Jay D. Keasling
- Joint BioEnergy Institute
- DOI
- https://doi.org/10.1038/s41467-020-16815-3
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
Hydroxyglutarate synthase (HglS) converts 2-oxoadipate to D-2- hydroxyglutarate during lysine catabolism in bacteria. Here the authors use structural and biochemical approaches to show that HglS acts via successive decarboxylation and intramolecular hydroxylation and that homologous enzymes catalyze the final step of lysine catabolism in plants.
