Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)

Matthew Alonzo; Javier Contreras; Shiqiao Ye; Hui Lin; Lumariz Hernandez-Rosario; Kim L. McBride; Karen Texter; Vidu Garg; Ming-Tao Zhao

Stem Cell Research (Oct 2022)

Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)

Matthew Alonzo,
Javier Contreras,
Shiqiao Ye,
Hui Lin,
Lumariz Hernandez-Rosario,
Kim L. McBride,
Karen Texter,
Vidu Garg,
Ming-Tao Zhao

Affiliations

Matthew Alonzo: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Javier Contreras: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Shiqiao Ye: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Hui Lin: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Lumariz Hernandez-Rosario: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA
Kim L. McBride: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA
Karen Texter: The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA
Vidu Garg: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA
Ming-Tao Zhao: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA; Corresponding author at: Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA.

Journal volume & issue: Vol. 64
p. 102892

Abstract

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Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect characterized by underdeveloped structures on the left side of the heart, including hypoplasia of the left ventricle and stenosis or atresia of the aortic and mitral valves. Here, we generated an iPSC line from the peripheral blood mononuclear cells of a male patient with HLHS through Sendai virus-mediated transfection of 4 Yamanaka factors. This iPSC line exhibited normal morphology, expressed pluripotency markers, had a normal karyotype, and could differentiate into cells of three germ layers. This iPSC line can be used for studying cellular and developmental etiologies of HLHS.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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