Multiplex imaging relates quantal glutamate release to presynaptic Ca2+ homeostasis at multiple synapses in situ

Thomas P. Jensen; Kaiyu Zheng; Nicholas Cole; Jonathan S. Marvin; Loren L. Looger; Dmitri A. Rusakov

doi:10.1038/s41467-019-09216-8

Nature Communications (Mar 2019)

Multiplex imaging relates quantal glutamate release to presynaptic Ca2+ homeostasis at multiple synapses in situ

Thomas P. Jensen,
Kaiyu Zheng,
Nicholas Cole,
Jonathan S. Marvin,
Loren L. Looger,
Dmitri A. Rusakov

Affiliations

Thomas P. Jensen: UCL Queen Square Institute of Neurology, University College London, Queen Square
Kaiyu Zheng: UCL Queen Square Institute of Neurology, University College London, Queen Square
Nicholas Cole: UCL Queen Square Institute of Neurology, University College London, Queen Square
Jonathan S. Marvin: Janelia Research Campus, Howard Hughes Medical Institute
Loren L. Looger: Janelia Research Campus, Howard Hughes Medical Institute
Dmitri A. Rusakov: UCL Queen Square Institute of Neurology, University College London, Queen Square

DOI: https://doi.org/10.1038/s41467-019-09216-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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How neurotransmitter release relates to presynaptic calcium dynamics is not fully understood. Here the authors develop an approach based on FLIM and optical quantal analysis for simultaneous imaging of calcium dynamics and glutamate release at presynaptic boutons.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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