Salivary melatonin is depleted in patients with dental caries due to the elevated oxidative stress

Abstract

Objectives: To determine salivary melatonin and malondialdehyde levels in individuals with and without dental caries. Materials and methods: Saliva samples were collected in a fasting state from patients with active dental caries (n ​= ​16) and patients without dental caries (n ​= ​16). Melatonin was measured in the samples using a commercially available ELISA kit and malondialdehyde was assayed using a standardized spectrophotometric method. Results: The salivary melatonin levels were significantly lower (p ​< ​0.01) in patients with active dental caries than patients without dental caries, while the salivary malondialdehyde values were significantly higher (p ​< ​0.01) in patients with active dental caries than patients without dental caries. The binary logistic regression analysis revealed a negative correlation (−0.513) between the salivary melatonin and malondialdehyde levels which was statistically significant (p ​< ​0.042) in the patient group with active dental caries, while no such relationship could be demonstrated in the patient group without dental caries. Conclusion: Melatonin depletion and augmented malondialdehyde levels potentially indicate that the endogenous melatonin has been utilized to counter the oxidative stress-induced during the initiation and progression of dental caries. Further research could explore the potential use of exogenous melatonin supplementation as a preventive and therapeutic measure for dental caries.

Keywords

• Dental caries
• Malondialdehyde
• Melatonin
• Oxidative stress
• Saliva