Nature Communications (Jan 2021)

A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF

  • Daniel J. Lightwood,
  • Rebecca J. Munro,
  • John Porter,
  • David McMillan,
  • Bruce Carrington,
  • Alison Turner,
  • Anthony Scott-Tucker,
  • Elizabeth S. Hickford,
  • Antje Schmidt,
  • David Fox,
  • Alison Maloney,
  • Tom Ceska,
  • Tim Bourne,
  • James O’Connell,
  • Alastair D. G. Lawson

DOI
https://doi.org/10.1038/s41467-020-20825-6
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

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TNF can be inhibited by small molecules that stabilize the TNF trimer in an asymmetric conformation. Here, the authors develop a monoclonal antibody that selectively binds this inactive form of TNF, enabling both target engagement assessment and structural characterization of TNF binding to TNF receptor 1.