Extraction of Environment-Friendly Biodegradable Poly-Hydroxy Butyrate Using Novel Hydrodynamic Cavitation Method

Abstract

Polyhydroxy butyrate (PHB) is one of the best environment-friendly bioplastic alternatives for petroleum-based plastic due to its biodegradability. However, it has less commercial popularity owing to the high cost of downstream processing that involves repeated centrifugation and the use of costly harmful solvents, as well as a labor-intensive process. Hydrodynamic Cavitation (HC) offers easy and simple mechanisms for downstream processing. Also, biopolymer extracted for haloarchea show an advantage of least contamination under the halophilic condition on an industrial level. In this paper, a haloarchaeal consortium producing biopolymer isolated from commercial rock salt has been subjected to HC as well as distilled water lysis. A maximum of 23 g.L-1 PHB was extracted in 40 min run with 50 passes and 0.10 cavitation number at 3.9 bar pressure. The extracted biopolymer was characterized and was found to be PHB. Comparative analysis shows that HC results in a substantial reduction in the downstream processing time. Moreover, it has double the efficiency of PHB extraction as compared to the distilled water lysis method. This paper reports the HC process as a techno-commercial alternative to industrial PHB extraction.

Keywords

• polyhydroxy butyrate, hydrodynamic cavitation, haloarchaea, biopolymers