Disulfide-compatible phage-assisted continuous evolution in the periplasmic space

Mary S. Morrison; Tina Wang; Aditya Raguram; Colin Hemez; David R. Liu

doi:10.1038/s41467-021-26279-8

Nature Communications (Oct 2021)

Disulfide-compatible phage-assisted continuous evolution in the periplasmic space

Mary S. Morrison,
Tina Wang,
Aditya Raguram,
Colin Hemez,
David R. Liu

Affiliations

Mary S. Morrison: Merkin Institute of Transformative Technologies in Health Care, Broad Institute of Harvard and MIT
Tina Wang: Merkin Institute of Transformative Technologies in Health Care, Broad Institute of Harvard and MIT
Aditya Raguram: Merkin Institute of Transformative Technologies in Health Care, Broad Institute of Harvard and MIT
Colin Hemez: Merkin Institute of Transformative Technologies in Health Care, Broad Institute of Harvard and MIT
David R. Liu: Merkin Institute of Transformative Technologies in Health Care, Broad Institute of Harvard and MIT

DOI: https://doi.org/10.1038/s41467-021-26279-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

Read online

The directed evolution of antibodies yields important tools for research and therapy. Here the authors develop a periplasmic phage-assisted continuous evolution platform for improvement of protein-protein interactions in the disulfidecompatible E. coli periplasm.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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