Nature Communications (Oct 2021)

Disulfide-compatible phage-assisted continuous evolution in the periplasmic space

  • Mary S. Morrison,
  • Tina Wang,
  • Aditya Raguram,
  • Colin Hemez,
  • David R. Liu

DOI
https://doi.org/10.1038/s41467-021-26279-8
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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The directed evolution of antibodies yields important tools for research and therapy. Here the authors develop a periplasmic phage-assisted continuous evolution platform for improvement of protein-protein interactions in the disulfidecompatible E. coli periplasm.