Effect of Ivermectin on the Expression of P-Glycoprotein in Third-Stage Larvae of <i>Haemonchus contortus</i> Isolated from China
Xiaoping Luo,
Shuyi Wang,
Ying Feng,
Penglong Wang,
Gaowa Gong,
Tianlong Guo,
Xingang Feng,
Xiaoye Yang,
Junyan Li
Affiliations
Xiaoping Luo
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China
Shuyi Wang
Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot 010031, China
Ying Feng
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China
Penglong Wang
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China
Gaowa Gong
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China
Tianlong Guo
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China
Xingang Feng
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Xiaoye Yang
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
Junyan Li
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010030, China
Haemonchus contortus poses a severe hazard to the healthy development of the sheep industry and threatens the welfare of sheep. Ivermectin is the primary anthelmintic used for the prevention and treatment of H. contortus parasitism. However, the widespread and uncontrolled application of ivermectin has resulted in the development and spread of resistant strains of H. contortus. P-glycoprotein (P-gp) plays important roles in the pharmacology and toxicology of ivermectin, and changes in P-gp expression levels can be used to analyze the resistance of H. contortus to ivermectin. This study aimed to analyze the effects of ivermectin on P-gp expression in H. contortus L3 larvae isolated from China and to evaluate whether changes in P-gp expression levels can be used to analyze resistant H. contortus strains. In the absence of drug treatment, the ivermectin-resistant strains isolated in China showed increased expression of P-gp11 (p P-gp2 and P-gp9.1 were downregulated (p P-gp expression was found to be very complex among the L3 larvae of different strains. In addition, it was confirmed that using P-gp to determine ivermectin resistance in H. contortus strains from different geographic environments can yield different results.
