Nature Communications (Jun 2020)

Ultra-large chemical libraries for the discovery of high-affinity peptide binders

  • Anthony J. Quartararo,
  • Zachary P. Gates,
  • Bente A. Somsen,
  • Nina Hartrampf,
  • Xiyun Ye,
  • Arisa Shimada,
  • Yasuhiro Kajihara,
  • Christian Ottmann,
  • Bradley L. Pentelute

DOI
https://doi.org/10.1038/s41467-020-16920-3
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Synthetic peptide libraries can access broad chemical space, but generally examine only ~ 106 compounds. Here, the authors show that in-solution affinity selection, interfaced with nLC-MS/MS sequencing, can identify binders from fully randomized synthetic libraries of 108 members.