Biology (Feb 2014)

In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

  • Thi-My-Nhung Hoang,
  • Hong-Lien Vu,
  • Ly-Thuy-Tram Le,
  • Chi-Hung Nguyen,
  • Annie Molla

DOI
https://doi.org/10.3390/biology3010167
Journal volume & issue
Vol. 3, no. 1
pp. 167 – 175

Abstract

Read online

Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.

Keywords