Cukurova Medical Journal (Dec 2020)
Redox modulatory effects of testosterone administration in liver tissue of aging rats
Abstract
Purpose: The aim of this sudy was to evaluate if testosterone administration possesses any ameliorative effects on age-related redox imbalance in liver tissue of male rats. Materials and Methods: Our study consists of three groups of male rats (n=24). Group 1: Young controls and Group 2: Aged rats that were administrated peanut oil intramuscularly andGroup 3: Aged rats that were administered a single dose of testosterone enanthate (25 mg/kg body weight) in peanut oil intramuscularly as a vehicle. To determine oxidative damage and also antioxidative capacity, we analyzed the levels of protein carbonyl groups, advanced oxidation end products, dityrosine, kynurenine, lipid hydroperoxides, advanced glycation end products, total thiol fractions, and Cu, Zn-superoxide dismutase activities. Results: The current study results indicate that redox balance is severely impaired in aged liver tissue via higher levels of oxidative protein damage, increased rate of lipid peroxidation, and also reduced antioxidant defense capacity. the present study showed that, testosterone administrationpartially ameliorated impaired redox balance. Conclusion: Testosterone administration partially improved impaired redox status by alleviating macromolecular oxidative damage and increase antioxidant defense capacity but these ameliorative effects not sufficient as young controls.
