Preparation of ferrocene core dendrimers and immobilization of AChE for detection of diclofop-methyl herbicide

Abstract

The aim of this work was to investigate diclofop-methyl detection (found in a variety of herbicides) using novel dendrimers that have ferrocene cores, as well as their Pt(II) and Pt(IV) complexes. Novel dendrimer structures were synthesized and characterized by molar conductivity, magnetic susceptibility, FTIR, UV-Vis, 1H-NMR and LC-MS methods. Then, the AChE (acetylcholine esterase) enzyme was immobilized on the novel dendrimers, and the optimal parameters (pH, temperature, repeated use, storage stability, substrate concentration) were determined for immobilized AChE. Lastly, changes in absorbance intensity were measured, and calibration graphs were plotted that reflect the inhibition reaction of immobilized AChE with diclofop-methyl. The inhibition interaction of the enzyme immobilized to the Pt(II) ion containing dendrimers was higher than that of the Pt(IV) ion containing dendrimers.

Keywords

• ferrocene, dendrimers, diclofopmethyl, acetylcholinesterase, immobilization