Characterization of Shrimp Oil from Pandalus borealis by High Performance Liquid Chromatography and High Resolution Mass Spectrometry
Guangling Jiao,
Joseph P. M. Hui,
Ian W. Burton,
Marie-Hélène Thibault,
Claude Pelletier,
Josée Boudreau,
Nadia Tchoukanova,
Balaji Subramanian,
Yahia Djaoued,
Stephen Ewart,
Jacques Gagnon,
Kathryn Vanya Ewart,
Junzeng Zhang
Affiliations
Guangling Jiao
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada
Joseph P. M. Hui
Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada
Ian W. Burton
Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada
Marie-Hélène Thibault
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada
Claude Pelletier
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada
Josée Boudreau
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada
Nadia Tchoukanova
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada
Balaji Subramanian
Université de Moncton, Campus de Shippagan, 218, boulevard J.-D.-Gauthier, Shippagan, NB E8S 1P6, Canada
Yahia Djaoued
Université de Moncton, Campus de Shippagan, 218, boulevard J.-D.-Gauthier, Shippagan, NB E8S 1P6, Canada
Stephen Ewart
Novaceutics Consulting, 6501 Oak St., Halifax, NS B3L 1H5, Canada
Jacques Gagnon
Coastal Zones Research Institute Inc., 232B, avenue de l'Église, Shippagan, NB E8S 1J2, Canada
Kathryn Vanya Ewart
Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada
Junzeng Zhang
Natural Health Products Program, Aquatic and Crop Resource Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada
Northern shrimp (Pandalus borealis) oil, which is rich in omega-3 fatty acids, was recovered from the cooking water of shrimp processing facilities. The oil contains significant amounts of omega-3 fatty acids in triglyceride form, along with substantial long-chain monounsaturated fatty acids (MUFAs). It also features natural isomeric forms of astaxanthin, a nutritional carotenoid, which gives the oil a brilliant red color. As part of our efforts in developing value added products from waste streams of the seafood processing industry, we present in this paper a comprehensive characterization of the triacylglycerols (TAGs) and astaxanthin esters that predominate in the shrimp oil by using HPLC-HRMS and MS/MS, as well as 13C-NMR. This approach, in combination with FAME analysis, offers direct characterization of fatty acid molecules in their intact forms, including the distribution of regioisomers in TAGs. The information is important for the standardization and quality control, as well as for differentiation of composition features of shrimp oil, which could be sold as an ingredient in health supplements and functional foods.
