The results of virological surveillance for intrauterine infections in Saint-Petersburg

Abstract

The purpose of researchis the determiningthe etiological structure ofintrauterine infections in Saint-Petersburg pediatric patients, pregnant women andinfants born to them using a variety of virological methods.Methods: serum from 164 children aged from 1 month to 14 years with diagnosis of «intrauterine infection». Serum from 80 pregnant women, collected in each trimester (total – 240 samples, their 42 children (at the age of 1–2 and 4–6 months of life, total – 82 samples). Immunoglobulin Mand G (IgM and IgG)to herpes virus type 1, cytomegalovirus (CMV), Toxoplasma gondii, mycoplasma and chlamydia, rubella, Epstein-Barr virus (EBV) and parvovirus B19, as well as IgG avidity,were determined by ELISA in all these samples. Theimmunoblot (Western blot), using the «Immunoblot2000» with test kits from «Euroimmun AG» (Germany), was applied to confirm cases. Statistical analysis wasperformed with theprograms Microsoft Excel, Statistica6.Results: cytomegalovirus, herpes virus 1st type and Epstein-Barr virus infections are dominate in the structure of intrauterine ones (45%, 23% and 14%, respectively). Laboratory evidence ofreactivationof cytomegalovirus (35% ofpregnant womenin the 2nd and/or 3rd trimesters) and acuteparvovirus infection (15% of cases) were found. Specific IgM to cytomegalovirus were detected in 6,2% ofchildren in the firstsix months of life.Conclusions: with the aim of early detection of cytomegalovirus reactivation and acute parvovirus infection it isnecessary to monitor pregnant women with the definition of specific IgM, IgG and avidity IgG. The procedure to using immunoblotting in the diagnosis of intrauterine infectionsneeds to be further study.

Keywords

• внутриутробные инфекции
• диагностика
• иммуноферментный анализ
• иммуноблоттинг