Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI

Abstract

Objective To investigate the clinical significance of the ratio of hsa_circ_0001708 to IKZF1 in bone marrow mononuclear cells (BMNC) in pediatric B-cell acute lymphoblastic leukemia (B-ALL). Methods T-A clone was used to construct the recombinant plasmid containing the back-spliced junction of hsa_circ_0001708, regarding the gradient diluted recombinant plasmid as standard substance to establish the absolute fluorescence quantitative PCR (qPCR) for the detection of hsa_circ_0001708. Bone marrow samples from 159 newly diagnosed pediatric B-ALL patients and 17 patients with non-malignant hemotologic diseases in their matched control group admitted in the Children's Hospital of Chongqing Medical University from January 2016 to January 2019. The copy number of hsa_circ_0001708 and IKZF1 in BMNC of B-ALL children were determined by absolute fluorescence qPCR. The patients were divided into different groups according to their age, gender, peripheral blood leukocyte count, molecular biology, genetics, immune classification, and whether relapse and survive during the follow-up period. The ratio of hsa_circ_0001708 to IKZF1 in different groups and the correlation with these clinical characteristics were analyzed retrospectively. Results The absolute RT-qPCR was established that can accurately quantify the copy number of hsa_circ_0001708. The ratio of hsa_circ_0001708 to IKZF1 was significantly increased in pediatric B-ALL (P < 0.01) when compared with the controls, and the area under the ROC curve (AUC) was 0.793 6. The correlation analysis indicated that the ratio was significant increased in these groups of high leukocyte count, high blast cell count of peripheral blood, pre-B and poor prognosis in cytogenetics (P < 0.05). Conclusion The ratio of hsa_circ_0001708 to IKZF1 has potential diagnostic value and is related to the MIC classification for pediatric B-ALL.

Keywords

• hsa_circ_0001708
• b-cell acute lymphoblastic leukemia
• diagnosis