Gayana: Botanica (Jan 2003)

PRODUCCION DE PIGMENTOS Y PROTEINAS DE LA CIANOBACTERIA ANABAENA PCC 7120 EN RELACION A LA CONCENTRACION DE NITROGENO E IRRADIANCIA PIGMENT AND PROTEIN PRODUCTION OF THE CYANOBACTERIUM ANABAENA PCC 7120 IN RELATION TO NITROGEN CONCENTRATION AND IRRADIANCE

  • César Loreto,
  • Néstor Rosales,
  • José Bermúdez,
  • Ever Morales

Journal volume & issue
Vol. 60, no. 2
pp. 83 – 89

Abstract

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Algunas cianobacterias del género Anabaena son productoras de pigmentos de interés comercial. Este artículo documenta el efecto de la concentración de nutrientes en función del NaNO3 a 0, 1, 2, 4 y 8 mM y de la irradiancia a 78, 156 y 238 µmol m-2 s-1, sobre el contenido de proteínas y pigmentos de Anabaena PCC 7120 en cultivos discontinuos. El crecimiento de la cianobacteria fue poco afectado por la concentración de nitrato, sin diferencia significativa entre 1 y 8 mM (p Cyanobacteria of the genus Anabaena produce pigments of commercial value. The present work reports the effect of nitrogen nutrient concentration (0, 1, 2, 4, and 8 mM NaNO3) and irradiance (78, 156 and 238 µmol m-2 s-1 ) on growth, pigment and protein content in Anabaena PCC 7120 in batch cultures. Growth of the cyanobacterium was little affected by nitrate concentration, with no significant differences between 1 and 8 mM (p< 0,05). However, a concentration of 8 mM gave rise to the highest levels of phycocyanin (174 ± 2 µg ml-1), chlorophyll a (17.4 ± 1.2 µg ml-1), protein (563 ± 2 µg ml-1) and carotenoids (4.5 ± 0.3 µg ml-1). Although cell densities were highest (117 (± 11) x10(6) cell ml-1) at 78 µmol m-2 s-1, maximum levels of protein and pigments were achieved at 156 µmol m-2 s-1. The number of heterocystes per trichome decreased with increasing nutrient concentration. Biomass production of Anabaena PCC 7120 enriched with phycocyanin, chlorophyll a, and protein was enhanced in a saturating-nutrient medium and a irradiance between 78 and 156 µmol m-2 s-1

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