Derivation of two induced pluripotent stem cell lines from a healthy control subject

Dan Zhang; Di Huang; Leon M. Larcher; Khine Zaw; Shang-Chih Chen; Luke Jennings; Tina M. Lamey; Jennifer A. Thompson; Terri L. McLaren; Fred K. Chen; Samuel McLenachan

Stem Cell Research (Feb 2025)

Derivation of two induced pluripotent stem cell lines from a healthy control subject

Dan Zhang,
Di Huang,
Leon M. Larcher,
Khine Zaw,
Shang-Chih Chen,
Luke Jennings,
Tina M. Lamey,
Jennifer A. Thompson,
Terri L. McLaren,
Fred K. Chen,
Samuel McLenachan

Affiliations

Dan Zhang: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia
Di Huang: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia
Leon M. Larcher: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia
Khine Zaw: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia
Shang-Chih Chen: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia
Luke Jennings: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia
Tina M. Lamey: Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia
Jennifer A. Thompson: Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia
Terri L. McLaren: Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia
Fred K. Chen: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia; Department of Ophthalmology, Royal Perth Hospital, Perth, Western Australia, Australia; Department of Ophthalmology, Perth Children’s Hospital, Nedlands, Western Australia, Australia; Corresponding authors.
Samuel McLenachan: Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Corresponding authors.

Journal volume & issue: Vol. 82
p. 103621

Abstract

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Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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