Disc electrophoresis of rat plasma lipoproteins

Abstract

The disc electrophoresis of lipoproteins of unfractionated rat plasma is described. The plasma was prestained with Sudan black B and electrophoretically separated at polyacrylamide gel concentrations of 7.5, 5, and 3.75%. At least four lipoprotein components were observed, and an additional 2–3 components in the main gel and 2–5 components in the spacer gel possibly were present. Densitometry of the resolved gel patterns indicated good reproducibility. Thin-layer chromatography of lipids extracted from the Sudan black B-binding components confirmed the lipoprotein nature of these components of rat plasma.A comparison of the disc electrophoretic patterns of human serum and rat plasma suggested that the low-density lipoprotein components of rat plasma are smaller in size than those of human serum.

Keywords

• electrophoresis
• disc
• acrylamide gel
• plasma
• lipoproteins
• rat