Genetic Diversity Among Independent Isolates of the <i>Dolichocephalovirinae</i> Subfamily

Abstract

Members of the Dolichocephalovirinae subfamily are giant viruses with an elongated head and a flexible tail that is used to infect Caulobacter strains. In this paper, we describe the isolation and characterization of nine newly isolated phages and present evidence that seven of these phages represent a new Dolichocephalovirinae genus that has significant differences from the four previously described Dolichocephalovirinae genera. In addition, since these new phages were isolated from a single sampling site over the course of three years, a comparison of their genome sequences reveals a low level of within-population diversity resulting from both single-nucleotide polymorphisms and insertions or deletions. A comparison of the host ranges of these phages suggests that differences in host susceptibility may be an important factor in maintaining this diversity.

Keywords

• <i>Caulobacter</i>
• Cbk-like phages
• genomic diversity
• phage–bacteria interactions
• broad host range bacteriophages
• <i>Dolichocephalovirinae</i>