Centre for Micro-photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia Peter MacCallum Cancer Research Centre, Parkville, Melbourne, Victoria, Australia
Jay Gilbert
Department of Food Science, Purdue University, West Lafayette, USA
Owen Jones
Department of Food Science, Purdue University, West Lafayette, USA
Nicholas Reynolds
ARC Training Centre for Biodevices, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia
Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved differentiation. Here we describe a detailed protocol for fabricating amyloid fibril networks suitable for eukaryotic cell culture applications.
