Initial study on removing cellular residues from hydrostatic high-pressure treated allogeneic tissue using ultrasound

Abstract

Hydrostatic high-pressure technology (HHD) devitalizes tissue quickly and gently, without negatively affecting the structural properties. HHD-treated tissues must be cleaned from devitalized cells. A partially automated, gentle, reproducible and timesaving rinsing test setup utilizing ultrasound is demonstrated in this study. The test setup is used to clean HHD-treated bone allografts of tissue residues and prevent microbiological contamination. A rinsing procedure is investigated. Residual DNA content determination is utilized to analyze cleaned bone allograft tissue for rinsing procedure evaluation.

Keywords

• hhd
• allograft
• cell residue
• decellularization
• rinsing device
• ultrasound
• test setup
• bone tissue