Проблемы особо опасных инфекций (Jun 2014)

Usage of chi-Sequence for Differentiation between the Strains of Anthrax Etiological Agent and Closely Related Bacilli

  • N. A. Shishkova,
  • A. N. Mokrievich,
  • V. M. Pavlov,
  • L. I. Marinin,
  • G. M. Vakhrameeva,
  • T. Yu. Kudryavtseva,
  • I. A. Dyatlov

DOI
https://doi.org/10.21055/0370-1069-2014-2-97-100
Journal volume & issue
Vol. 0, no. 2
pp. 97 – 100

Abstract

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Objective of the study was to carry out comparison between the microorganisms appurtenant to six different species of Bacilli genus as regards mobility of the amplicons obtained using the primer that contained chi-sequence of Bacillus subtilis . Therewith, analyzed were 30 Bacillus anthracis strains and closely related bacilli. Cultures grew on L-agar and L-broth at 37 °C. DNA was isolated from vegetative B. anthracis and Bacillus spp . cultures introducing lysozyme and phenol de-proteinizing. In order to perform single-primer PCR constructed was ChiBs primer - 5’-CTAGGAGCGGG-3’. Single-primer amplification (30 cycles) was carried out at annealing temperature of 47 °C. Comparative analysis of individual electrophoretic amplicon profiles, obtained by means of ChiBs-primer PCR, allows for identification of genetic intraspecific variability and differentiation of B. anthracis strains from the atypical ones and closely related species of bacilli.

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