Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets

Charalampos Kapsalis; Bolin Wang; Hassane El Mkami; Samantha J. Pitt; Jason R. Schnell; Terry K. Smith; Jonathan D. Lippiat; Bela E. Bode; Christos Pliotas

doi:10.1038/s41467-019-12591-x

Nature Communications (Oct 2019)

Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets

Charalampos Kapsalis,
Bolin Wang,
Hassane El Mkami,
Samantha J. Pitt,
Jason R. Schnell,
Terry K. Smith,
Jonathan D. Lippiat,
Bela E. Bode,
Christos Pliotas

Affiliations

Charalampos Kapsalis: Biomedical Sciences Research Complex, School of Biology, University of St Andrews
Bolin Wang: Biomedical Sciences Research Complex, School of Biology, University of St Andrews
Hassane El Mkami: School of Physics and Astronomy, University of St Andrews
Samantha J. Pitt: School of Medicine, University of St Andrews
Jason R. Schnell: Department of Biochemistry, University of Oxford
Terry K. Smith: Biomedical Sciences Research Complex, School of Chemistry, University of St Andrews
Jonathan D. Lippiat: School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds
Bela E. Bode: Centre of Magnetic Resonance, University of St Andrews
Christos Pliotas: Biomedical Sciences Research Complex, School of Biology, University of St Andrews

DOI: https://doi.org/10.1038/s41467-019-12591-x
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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How mechanosensitive ion channels, such as MscL, are activated by lipids and physical properties of the membrane remains unclear. Here authors use PELDOR/DEER spectroscopy and identify a single site which generated an allosteric structural response in the absence of membrane tension.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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