Semi conductors with nano-structure play an important role in detecting and destroying harmful chemical contaminants such as dye. In this study, the effective parameters including coating method, initial pH, dye concentration, energy consumption, kind and intensity of UV lamp in AB113 dye removal by photo-catalytic process using TiO2 nano-particles immobilized on concrete bed were studied. The results have shown that the Separated Sealer and Photocatalyst (SSP) method had higher efficiency. According to the results, over 92 percent of 75 ppm AB113 and 17, 6.7 and 9.3 percent of benezylic, naphthalene and phenolic rings and 21 percent of TOC were removed using 40 gr/m2 immobilized TiO2 and 90 W UV-C lamps at pH 9 in less than 5 hours.