Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline
Vadim I. Chechekhin,
Konstantin Yu. Kulebyakin,
Natalia I. Kalinina,
Pyotr A. Tyurin-Kuzmin
Affiliations
Vadim I. Chechekhin
Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, Russia
Konstantin Yu. Kulebyakin
Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, Russia
Natalia I. Kalinina
Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, Russia
Pyotr A. Tyurin-Kuzmin
Corresponding author.; Department of Biochemistry and Regenerative Biomedicine, Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, Russia
Stem and progenitor cells are characterized by peculiar mechanisms of hormonal regulation. Here we describe a protocol of analysis of hormonal cross-talk in adipose tissue derived multipotent mesenchymal stem cells (MSCs). Specifically, cells were treated by a “sensitizing” hormone/neuromediator followed by the measurement of cellular Ca2+ response to the “readout” hormone after various time intervals. This protocol was successfully used in studies demonstrating a permissive effect of noradrenaline and 5-HT on MSCs sensitivity to noradrenaline, which is a predictive marker of the development of obesity-associated arterial hypertension.
