Fluorescent Antibody Technique For Detection Of Salmonella Typhimurium In The Intestine Of Experimentally Infected Calves

Abstract

A systemic study on the pathogenesis of experimentally induced Salmonella typhimurium infection was carried out in calves. Sixteen normal, colostrum-fed, friesian calves, ranging in age from 3 to 6 weeks were used. The calves were divided into two equal groups. Group I calves were inoculated orally with 1.5 x 1011 CFU of Salmonella typhimurium and group II calves served as control. Using an indirect fluorescent antibody technique, it was found that Salmonella typhimurium has an apparent predilection for the small intestine particularly jejunum and ileum. The results of this study suggest that this technique can be considered as a rapid reliable technique for diagnosis of salmonellosis in endemic outbreaks.