Anti-Inflammatory and Antioxidant Activity of Pollen Extract Collected by <i>Scaptotrigona affinis postica</i>: in silico, in vitro, and in vivo Studies
Alberto Jorge Oliveira Lopes,
Cleydlenne Costa Vasconcelos,
João Batista Santos Garcia,
Myssa Sued Dória Pinheiro,
Francisco Assis Nascimento Pereira,
Darleno de Sousa Camelo,
Sebastião Vieira de Morais,
José Roberto Brito Freitas,
Cláudia Quintino da Rocha,
Maria Nilce de Sousa Ribeiro,
Maria do Socorro de Sousa Cartágenes
Affiliations
Alberto Jorge Oliveira Lopes
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
Cleydlenne Costa Vasconcelos
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
João Batista Santos Garcia
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
Myssa Sued Dória Pinheiro
Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, Brazil
Francisco Assis Nascimento Pereira
Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, Brazil
Darleno de Sousa Camelo
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
Sebastião Vieira de Morais
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
José Roberto Brito Freitas
Center for Agricultural and Environmental Sciences, Campus IV, Federal University of Maranhão, Chapadinha 65500-000, MA, Brazil
Cláudia Quintino da Rocha
Chemistry Department, Federal University of Maranhão, São Luís 65080-805, Brazil
Maria Nilce de Sousa Ribeiro
Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, Brazil
Maria do Socorro de Sousa Cartágenes
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
Bees are of great importance for plant diversity for being an important pollinating agents. Stingless bees such as Scaptotrigona affinis postica, is cultivated largely due to the products offered by it. Pollen is one of these products, which has been highlighted for exhibit various therapeutic properties. Considering the bioactivity of this natural product, this study investigated the antioxidant, anti-inflammatory, antinociceptive activities, and elucidated the chemical composition of pollen collected extract by Scaptotrigona affinis postica. Using in vitro assays, the antioxidant potential and inhibitory activity against the COX enzyme from pollen extract was evaluated. Additionally, tests were performed to measure the anti-inflammatory and antinociceptive activities in animal models. In our results, we found that pollen extract showed antioxidant effects and inhibitory activity against the COX enzyme. The in vivo assays showed that the extract acts on the nervous system in local and systemic levels and that the anti-inflammatory activity is due the prostanoids reducing. Chemical analyses recognize 10 molecules in the extract belonging to the polyphenol and flavonoids classes and the computational study suggests that is responsible for the observed results. Thus, it is reported for the first time the biological potential of S. aff. postica pollen extract and we conclude that this bee product can be considered as one source of potential new drugs.
