A novel single-base mutation in CaSGR1 confers the stay-green phenotype in pepper

Yuhua Liu; Lijun Ou; Zhoubin Liu; Junheng Lyu; Jing Wang; Jingshuang Song; Bozhi Yang; Wenchao Chen; Sha Yang; Wei Liu; Xuexiao Zou; Zhuqing Zhang

Horticultural Plant Journal (Apr 2023)

A novel single-base mutation in CaSGR1 confers the stay-green phenotype in pepper

Yuhua Liu,
Lijun Ou,
Zhoubin Liu,
Junheng Lyu,
Jing Wang,
Jingshuang Song,
Bozhi Yang,
Wenchao Chen,
Sha Yang,
Wei Liu,
Xuexiao Zou,
Zhuqing Zhang

Affiliations

Yuhua Liu: College of Life Sciences and Environment, Hengyang Normal University, Hengyang, Henan 421008, China; Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, Chin
Lijun Ou: College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China
Zhoubin Liu: College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China
Junheng Lyu: Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
Jing Wang: Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
Jingshuang Song: Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
Bozhi Yang: College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China
Wenchao Chen: Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
Sha Yang: Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
Wei Liu: College of Life Sciences and Environment, Hengyang Normal University, Hengyang, Henan 421008, China; Corresponding authors.
Xuexiao Zou: College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, Chin; Corresponding authors.
Zhuqing Zhang: Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China; Corresponding authors.

Journal volume & issue: Vol. 9, no. 2
pp. 293 – 305

Abstract

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The stay-green trait is of considerable importance in extending the shelf life of green pepper fruit (Capsicum annuum L.) and in enhancing the appearance of ornamental plants. The study revealed the genetic and regulatory mechanisms of the stay-green trait in pepper, which will aid in the selection of ornamental pepper varieties. In this study, a pepper mutant with stay-green fruit named TNX348 was identified from a germplasm resource bank. Two segregating populations were constructed using the stay-green mutant TNX348 and then used in bulked segregant analysis combined with RNA sequencing and linkage analyses. The causal gene of the stay-green trait was mapped to an approximately 131-kb region, and a senescence-induced chloroplast protein gene, CaSGR1 (Capana01g000359), was identified as a candidate gene. Sequencing analysis revealed a G→A single-base mutation of CaSGR1 in TNX348 that led to early termination of translation. Based on the single-base mutation, a single nucleotide polymorphism (SNP) marker co-segregating with the stay-green trait was developed. Furthermore, in transcriptome analysis, expression patterns of 11 hormone transduction-related transcription factors, such as abscisic acid-insensitive (ABI), abscisic acid-responsive element-binding factor (ABF), and NAC transcription factor, were similar or opposite to that of CaSGR1. The results indicated that the transcription factors might mediate chlorophyll degradation by regulating the expression of CaSGR1.

Published in Horticultural Plant Journal

ISSN: 2095-9885 (Print); 2468-0141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Plant culture
Website: https://www.keaipublishing.com/en/journals/horticultural-plant-journal/

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