Online histological atlas of the Göttingen minipig brain
Dariusz Orlowski,
Andreas N. Glud,
Nicola Palomero-Gallagher,
Jens Christian H. Sørensen,
Carsten R. Bjarkam
Affiliations
Dariusz Orlowski
Center for Experimental Neuroscience (Cense), Institute of Clinical Medicine – The Department of Neurosurgery, Aarhus University, Aarhus Universitetshospital, Palle Juul-Jensens Boulevard 165, Indgang J, Plan 1, J118-125, DK-8200 Aarhus N, Denmark; Corresponding author.
Andreas N. Glud
Center for Experimental Neuroscience (Cense), Institute of Clinical Medicine – The Department of Neurosurgery, Aarhus University, Aarhus Universitetshospital, Palle Juul-Jensens Boulevard 165, Indgang J, Plan 1, J118-125, DK-8200 Aarhus N, Denmark
Nicola Palomero-Gallagher
Institute of Neuroscience and Medicine (INM-1), Research Centre Jülich, 52425 Jülich, Germany; Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, RWTH Aachen, Aachen, Germany
Jens Christian H. Sørensen
Center for Experimental Neuroscience (Cense), Institute of Clinical Medicine – The Department of Neurosurgery, Aarhus University, Aarhus Universitetshospital, Palle Juul-Jensens Boulevard 165, Indgang J, Plan 1, J118-125, DK-8200 Aarhus N, Denmark; Department of Neurosurgery, Aarhus University Hospital, Aarhus Universitetshospital, Palle Juul-Jensens Boulevard 165, Indgang J, Plan 6, DK-8200 Aarhus N, Denmark
Carsten R. Bjarkam
Department of Neurosurgery, Aalborg University Hospital, and Institute of Clinical Medicine, Aalborg University, Hobrovej 18-22, DK-9000 Aalborg, Denmark
Background: The cytoarchitecture of the Göttingen minipig telencephalon has recently been elucidated in the published article (Bjarkam et al., 2017). The aim of the current paper is to describe how such data can be presented in an online histological atlas of the Gottingen minipig brain and how this atlas was constructed. Methods: Two sets of histological sections were used. One set was photographed in high resolution and labelled, the other set in low resolution (resized first set) was used for reference on the computer screen. The two sets of microphotographs enable, using the freely available JQuery Image Zoom Plugin, the subsequent construction of a simple HTML-based atlas web page with a “virtual microscope like” style, which allowed magnifying of the base image (low-resolution image) up to the maximum resolution of the high-resolution image. In addition, we describe how the established histological atlas can be accompanied by a set of similar T1-weighted MRI pictures. Results and conclusion: Histological and MRI pictures are presented in atlas form on www.cense.dk/minipig_atlas/index.html. The described pipeline represent a cheap and freely available way to present histological images, in online virtual microscopic atlas form, and may thus be of general interest to anyone who would like to present histological data accordingly.
