Ветеринария сегодня (Apr 2018)

Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera

  • Ye. S. Kostrova,
  • O. P. Bjadovskaya,
  • Ye. V. Peshkova,
  • O. V. Pruntova

Journal volume & issue
Vol. 0, no. 1
pp. 34 – 37

Abstract

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Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined.

Keywords