The ecological and economical importance of the Galea spixii, a hystricognath cavid rodent, has aroused the interest of the researchers at developing studies focused on the conservation of its germplasm. In this sense, this review presents published information regarding methods for obtaining and characterizing both male and female gametes. Moreover, interesting data regarding sperm freezing and vitrification of testicular and ovarian tissue are shown. Such promising studies evidence the possibilities for improving other assisted reproductive techniques focused on G. spixii multiplication and conservation