The study of plant protein accumulation in gut of insect using proteomics technique: Wheat–sunn pest interaction

Abstract

Sunn pest, Eurygaster integriceps, is a serious pest of wheat and barley in Iran. The gut and salivary glands are main parts of digestive system in sunn pest. The performance of these organs in digestion is related to its’ expressed proteins. The use of proteomics technique to study plant protein behaviors in gut of insects is new method in insect–plant interaction experiments. In this study, some of plant protein spots were traced in adult gut of sunn pest using 2 DE, mass spectrometry and NCBI database. Six proteins contain serpin, β-amylase, α-amylase inhibitor, dehydrosacorbate reducyase, triticin and α-l arabinofuranidose were identified using plant database. The study of sunn pest–wheat interaction and identification of effective proteins in stability of this relation can be helpful for finding of new target proteins in pest control.

Keywords

• Sunn pest
• Gut
• Plant protein
• Proteomics
• Pest control