Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by <i>Neospora caninum</i>
Javier Regidor-Cerrillo,
Dong Xia,
Laura Jiménez-Pelayo,
Marta García-Sánchez,
Esther Collantes-Fernández,
Nadine Randle,
Jonathan Wastling,
Luis-Miguel Ortega-Mora,
Pilar Horcajo
Affiliations
Javier Regidor-Cerrillo
SALUVET-Innova, Faculty of Veterinary Sciences, Complutense University of Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain
Dong Xia
Department of Comparative Biomedical Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
Laura Jiménez-Pelayo
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
Marta García-Sánchez
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
Esther Collantes-Fernández
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
Nadine Randle
Department of Infection Biology, Institute of Infection & Global Health, University of Liverpool, Liverpool Science Park IC2, 146 Brownlow Hill, Liverpool L3 5RF, UK
Jonathan Wastling
Faculty of Natural Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
Luis-Miguel Ortega-Mora
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
Pilar Horcajo
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
Despite the importance of bovine neosporosis, relevant knowledge gaps remain concerning the pathogenic mechanisms of Neospora caninum. Infection of the placenta is a crucial event in the pathogenesis of the disease; however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3 in terms of proliferative capacity and transcriptome host cell modulation. Herein, we used the same model of infection to study the interaction of Neospora with these target cells at the proteomic level using LC-MS/MS over the course of the parasite lytic cycle. We also analysed the proteome differences between high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates. The results showed that mitochondrial processes and metabolism were the main points of Neospora-host interactions. Interestingly, Nc-Spain1H infection showed a higher level of influence on the host cell proteome than Nc-Spain7 infection.
