Nature Communications (Mar 2021)

Engineered yeast genomes accurately assembled from pure and mixed samples

  • Joseph H. Collins,
  • Kevin W. Keating,
  • Trent R. Jones,
  • Shravani Balaji,
  • Celeste B. Marsan,
  • Marina Çomo,
  • Zachary J. Newlon,
  • Tom Mitchell,
  • Bryan Bartley,
  • Aaron Adler,
  • Nicholas Roehner,
  • Eric M. Young

DOI
https://doi.org/10.1038/s41467-021-21656-9
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

Read online

The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.