Frontiers in Genetics (Jun 2020)
Quantitative Trait Locus Analysis of Protein and Oil Content in Response to Planting Density in Soybean (Glycine max [L.] Merri.) Seeds Based on SNP Linkage Mapping
- Xiaocui Tian,
- Xiaocui Tian,
- Xiaocui Tian,
- Kaixin Zhang,
- Kaixin Zhang,
- Kaixin Zhang,
- Shulin Liu,
- Xu Sun,
- Xu Sun,
- Xu Sun,
- Xiyu Li,
- Xiyu Li,
- Xiyu Li,
- Jie Song,
- Jie Song,
- Jie Song,
- Zhongying Qi,
- Zhongying Qi,
- Zhongying Qi,
- Yue Wang,
- Yue Wang,
- Yue Wang,
- Yanlong Fang,
- Yanlong Fang,
- Yanlong Fang,
- Jiajing Wang,
- Jiajing Wang,
- Jiajing Wang,
- Sitong Jiang,
- Sitong Jiang,
- Sitong Jiang,
- Chang Yang,
- Chang Yang,
- Chang Yang,
- Zhixi Tian,
- Wen-Xia Li,
- Wen-Xia Li,
- Wen-Xia Li,
- Hailong Ning,
- Hailong Ning,
- Hailong Ning
Affiliations
- Xiaocui Tian
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Xiaocui Tian
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Xiaocui Tian
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Kaixin Zhang
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Kaixin Zhang
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Kaixin Zhang
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Shulin Liu
- State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
- Xu Sun
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Xu Sun
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Xu Sun
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Xiyu Li
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Xiyu Li
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Xiyu Li
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Jie Song
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Jie Song
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Jie Song
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Zhongying Qi
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Zhongying Qi
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Zhongying Qi
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Yue Wang
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Yue Wang
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Yue Wang
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Yanlong Fang
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Yanlong Fang
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Yanlong Fang
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Jiajing Wang
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Jiajing Wang
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Jiajing Wang
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Sitong Jiang
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Sitong Jiang
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Sitong Jiang
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Chang Yang
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Chang Yang
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Chang Yang
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Zhixi Tian
- State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
- Wen-Xia Li
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Wen-Xia Li
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Wen-Xia Li
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- Hailong Ning
- Key Laboratory of Soybean Biology, Ministry of Education, Northeast Agricultural University, Harbin, China
- Hailong Ning
- Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China
- Hailong Ning
- Soybean Research Institute, Northeast Agricultural University, Harbin, China
- DOI
- https://doi.org/10.3389/fgene.2020.00563
- Journal volume & issue
-
Vol. 11
Abstract
Soybean varieties suitable for high planting density allow greater yields. However, the seed protein and oil contents, which determine the value of this crop, can be influenced by planting density. Thus, it is important to understand the genetic basis of the responses of different soybean genotypes to planting density. In this study, we quantified the protein and oil contents in a four-way recombinant inbred line (FW-RIL) soybean population under two planting densities and the response to density. We performed quantitative trait locus (QTL) mapping using a single nucleotide polymorphism (SNP) linkage map generated by inclusive composite interval mapping. We identified 14 QTLs for protein content and 17 for oil content at a planting density of 2.15 × 105 plant/ha (D1) and 14 QTLs for protein content and 20 for oil content at a planting density 3.0 × 105 plant/ha (D2). Among the QTLs detected, two oil-content QTLs was detected at both plant densities. In addition, we identified 38 QTLs for the responses of protein and oil contents to planting density. Of the QTLs detected, 70 were identified in previous studies, while 33 were newly identified. Fourty-five QTLs accounted for over 10% of the phenotypic variation of the corresponding trait, based on 23 QTLs at a marker interval distance of ~600 kb detected under different densities and with the responses to density difference. Pathway analysis revealed four candidate genes involved in protein and oil biosynthesis/metabolism. These results improve our understanding of the genetic underpinnings of protein and oil biosynthesis in soybean, laying the foundation for enhancing protein and oil contents and increasing yields in soybean.
Keywords
