A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue

Debora Lo Furno; Adriana Carol Eleonora Graziano; Rosanna Avola; Rosario Giuffrida; Vincenzo Perciavalle; Francesco Bonina; Giuliana Mannino; Venera Cardile

doi:10.1155/2016/4563815

PPAR Research (Jan 2016)

A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue

Debora Lo Furno,
Adriana Carol Eleonora Graziano,
Rosanna Avola,
Rosario Giuffrida,
Vincenzo Perciavalle,
Francesco Bonina,
Giuliana Mannino,
Venera Cardile

Affiliations

Debora Lo Furno: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy
Adriana Carol Eleonora Graziano: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy
Rosanna Avola: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy
Rosario Giuffrida: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy
Vincenzo Perciavalle: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy
Francesco Bonina: Department of Drug Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy
Giuliana Mannino: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy
Venera Cardile: Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, Italy

DOI: https://doi.org/10.1155/2016/4563815
Journal volume & issue: Vol. 2016

Abstract

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The aim of this research was to assess the impact of a well-characterized extract from Citrus bergamia juice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model. To evaluate the effects on adipogenesis, some cell cultures were treated with adipogenic medium plus 10 or 100 μg/mL of extract. To determine the properties on lipolysis, additional mesenchymal stem cells were cultured with adipogenic medium for 14 days and after this time added with Citrus bergamia for further 14 days. To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5′-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay. Citrus bergamia extract suppressed the accumulation of intracellular lipids in mesenchymal stem cells during adipogenic differentiation and promoted lipolysis by repressing the expression of adipogenic genes and activating lipolytic genes. Citrus bergamia extract could be a useful natural product for improving adipose mobilization in obesity-related disorders.

Published in PPAR Research

ISSN: 1687-4757 (Print); 1687-4765 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://onlinelibrary.wiley.com/journal/1751

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