The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice
Hui Wang,
Zhaojun Chen,
Mei Wang,
Mingxiu Long,
Tingyuan Ren,
Chao Chen,
Xiaotong Dai,
Sheng Yang,
Shuming Tan
Affiliations
Hui Wang
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China
Zhaojun Chen
Institute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China
Mei Wang
Institute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China
Mingxiu Long
Guizhou Research Institute of Modern Agricultural Development, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China
Tingyuan Ren
Key Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
Chao Chen
Key Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
Xiaotong Dai
Key Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
Sheng Yang
Key Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
Shuming Tan
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China
Rosa roxburghii fruit is an underutilized functional food abundant in polyphenols. Polyphenols have been proved to have antidiabetic effects. This study investigates the effects of Rosa roxburghii fruit polyphenols extract (RPE) on plasma metabolites and gut microbiota composition in streptozotocin (STZ)- and high-fat diet- induced type 2 diabetes using metabolomics and 16S rRNA gene sequencing. The induced diabetic mice were fed with 400 mg/kg body weight RPE for 8 weeks. RPE demonstrated hypoglycemic, hypolipidemic, and anti-inflammatory effects. Colonic oxidative stress biomarkers were also lowered by RPE. Besides, RPE decreased plasma ceramides and tyrosine levels and increased carnitine and phosphatidylinositols levels, indicating improved insulin resistance, lipid metabolism, and immune response. Furthermore, RPE decreased abundances of Lachnospiraceae and Rikenellaceae and increased abundances of Erysipelotrichaceae and Faecalibaculum. Metabolic function prediction of the gut microbiota by PICRUSt demonstrated that RPE downregulated the phosphotransferase system. Taken together, these findings demonstrated that RPE has the potential to prevent type 2 diabetes by regulating the plasma metabolites and gut microbes.
