Sulfated Glycans Inhibit the Interaction of MERS-CoV Receptor Binding Domain with Heparin
Jiyuan Yang,
Yuefan Song,
Weihua Jin,
Ke Xia,
Grace C. Burnett,
Wanjin Qiao,
John T. Bates,
Vitor H. Pomin,
Chunyu Wang,
Mingqiang Qiao,
Robert J. Linhardt,
Jonathan S. Dordick,
Fuming Zhang
Affiliations
Jiyuan Yang
The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
Yuefan Song
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Weihua Jin
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
Ke Xia
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Grace C. Burnett
Department of Cell & Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216, USA
Wanjin Qiao
The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
John T. Bates
Department of Cell & Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216, USA
Vitor H. Pomin
Department of BioMolecular Sciences, Research Institute of Pharmaceutical Sciences, The University of Mississippi, Oxford, MS 38677, USA
Chunyu Wang
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Mingqiang Qiao
The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
Robert J. Linhardt
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Jonathan S. Dordick
Departments of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Fuming Zhang
Departments of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic virus with high contagion and mortality rates. Heparan sulfate proteoglycans (HSPGs) are ubiquitously expressed on the surface of mammalian cells. Owing to its high negatively charged property, heparan sulfate (HS) on the surface of host cells is used by many viruses as cofactor to facilitate viral attachment and initiate cellular entry. Therefore, inhibition of the interaction between viruses and HS could be a promising target to inhibit viral infection. In the current study, the interaction between the receptor-binding domain (RBD) of MERS-CoV and heparin was exploited to assess the inhibitory activity of various sulfated glycans such as glycosaminoglycans, marine-sourced glycans (sulfated fucans, fucosylated chondroitin sulfates, fucoidans, and rhamnan sulfate), pentosan polysulfate, and mucopolysaccharide using Surface Plasmon Resonance. We believe this study provides valuable insights for the development of sulfated glycan-based inhibitors as potential antiviral agents.
