Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture
Mengting Qi,
Meilin Ruan,
Jinjin Liang,
Zhengtao Zhang,
Chaohui Chen,
Yiping Cao,
Rongxiang He
Affiliations
Mengting Qi
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
Meilin Ruan
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
Jinjin Liang
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
Zhengtao Zhang
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
Chaohui Chen
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
Yiping Cao
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
Rongxiang He
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, China
A 3D network capture substrate based on poly(lactic-co-glycolic acid) (PLGA) nanofibers was studied and successfully used for high-efficiency cancer cell capture. The arc-shaped glass micropillars were prepared by chemical wet etching and soft lithography. PLGA nanofibers were coupled with micropillars by electrospinning. Given the size effect of the microcolumn and PLGA nanofibers, a three-dimensional of micro-nanometer spatial network was prepared to form a network cell trapping substrate. After the modification of a specific anti-EpCAM antibody, MCF-7 cancer cells were captured successfully with a capture efficiency of 91%. Compared with the substrate composed of 2D nanofibers or nanoparticles, the developed 3D structure based on microcolumns and nanofibers had a greater contact probability between cells and the capture substrate, leading to a high capture efficiency. Cell capture based on this method can provide technical support for rare cells in peripheral blood detection, such as circulating tumor cells and circulating fetal nucleated red cells.
