Dietary <i>Aspergillus oryzae</i> Modulates Serum Biochemical Indices, Immune Responses, Oxidative Stress, and Transcription of <i>HSP70</i> and Cytokine Genes in Nile Tilapia Exposed to Salinity Stress
Mustafa Shukry,
Marwa F. Abd El-Kader,
Basma M. Hendam,
Mahmoud A. O. Dawood,
Foad A. Farrag,
Salama Mostafa Aboelenin,
Mohamed Mohamed Soliman,
Hany M. R. Abdel-Latif
Affiliations
Mustafa Shukry
Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Marwa F. Abd El-Kader
Fish Diseases and Management, Sakha Aquaculture Research Unit, Central Laboratory for Aquaculture Research (A.R.C.), Cairo 11511, Egypt
Basma M. Hendam
Genetics and Genetic Engineering, Department of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Mahmoud A. O. Dawood
Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Foad A. Farrag
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Salama Mostafa Aboelenin
Biology Department, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Mohamed Mohamed Soliman
Clinical Laboratory Sciences Department, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Hany M. R. Abdel-Latif
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, Egypt
Nile tilapia Juveniles (19.50 ± 0.5 g) were fed on a basal diet (control group (CTR)) and a diet supplemented with 1 g Aspergillus oryzae (ASP) per kg diet for 12 weeks. Fish were then subjected to different salinity levels (0, 10, 15, and 20 practical salinity units (psu)) for another 15 days. Two-way ANOVA analysis revealed that the individual effects of ASP in Nile tilapia exposed to salinity levels presented a significant decrease (p p p < 0.05) downregulation of the mRNA expression values of heat shock protein 70 and interferon-gamma genes, alongside upregulation of the mRNA expression values of interleukin 1 beta and interleukin 8 genes, in the hepatic tissues of Nile tilapia in ASP-supplemented groups exposed to different salinities compared to those in the CTR group exposed to the same salinity levels. Taken together, these findings supported the potential efficacy of dietary supplementation with ASP in alleviating salinity stress-induced haemato-biochemical alterations, immune suppression, and oxidative stress in the exposed Nile tilapia.
