Nature Communications (Jul 2018)

Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID

  • Hiroki Sasaguri,
  • Kenichi Nagata,
  • Misaki Sekiguchi,
  • Ryo Fujioka,
  • Yukio Matsuba,
  • Shoko Hashimoto,
  • Kaori Sato,
  • Deepika Kurup,
  • Takanori Yokota,
  • Takaomi C. Saido

DOI
https://doi.org/10.1038/s41467-018-05262-w
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 8

Abstract

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CRISPR-guided cytidine deaminases, including BE3 (Base Editor 3) and Target-AID (activation-induced cytidine deaminase), can covert C:G base pairs to T:A at target site. Here, the authors generate missense mutations of mouse Psen1 gene and find BE3 has higher editing efficiency than Target-AID.