Reduced Staphylococcus aureus biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

Abstract

Si-feng Shi,1,2 Jing-fu Jia,3 Xiao-kui Guo,4 Ya-ping Zhao,3 De-sheng Chen,2 Yong-yuan Guo,2 Xian-long Zhang21Department of Orthopedics, the Affiliated Hospital of Xu Zhou Medical University, Xu Zhou, Jiangsu, 2Department of Orthopedic Surgery, Shanghai sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, 3School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai, 4Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China Abstract: Staphylococcus aureus can adhere to most foreign materials and form biofilm on the surface of medical devices. Biofilm infections are difficult to resolve. The goal of this in vitro study was to explore the use of chitosan-coated nanoparticles to prevent biofilm formation. For this purpose, S. aureus was seeded in 96-well plates to incubate with chitosan-coated iron oxide nanoparticles in order to study the efficiency of biofilm formation inhibition. The biofilm bacteria count was determined using the spread plate method; biomass formation was measured using the crystal violet staining method. Confocal laser scanning microscopy and scanning electron microscopy were used to study the biofilm formation. The results showed decreased viable bacteria numbers and biomass formation when incubated with chitosan-coated iron oxide nanoparticles at all test concentrations. Confocal laser scanning microscopy showed increased dead bacteria and thinner biofilm when incubated with nanoparticles at a concentration of 500 µg/mL. Scanning electron microscopy revealed that chitosan-coated iron oxide nanoparticles inhibited biofilm formation in polystyrene plates. Future studies should be performed to study these nanoparticles for anti-infective use. Keywords: chitosan-coated iron oxide nanoparticles, Staphylococcus aureus, biofilm

Keywords

• chitosn-coated iron oxide nanoparticles
• Staphylococcus aureus
• biofilm.