Broad-specific monoclonal antibody based IACs purification coupled UPLC-MS/MS method for T-2 and HT-2 toxin determination in maize and cherry samples

Abstract

In this work, broad-specific monoclonal antibody based immunoaffinity columns (IACs) coupled to ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for purification and determination of T-2 and HT-2 toxin in maize and cherry samples. Based on broad-specific anti-T-2 monoclonal antibody, the prepared IACs could be used for both T-2 and HT-2 purification with cross reactivity values at 100% for T-2 and 108% for HT-2. Column capacity was 3.14 μg mL−1 gel for T-2 toxin, and 3.20 μg mL−1 gel for HT-2 toxin. After 10 cycles of usage at intervals of 2 days, column capacity decreased to 0.85 μg mL−1 gel for T-2 and 1.22 μg mL−1 gel for HT-2 toxin. The developed method exhibited high sensitive results with limit of detection (LOD) lower than 0.35 μg kg−1 and 1.42 μg kg−1.

Keywords

• broad-specific immune-affinity columns
• t-2 toxin
• ht-2 toxin
• uplc-ms/ms