The effects of olibanum on male reproductive system damage in a lipopolysaccharide induced systemic inflammation model in rat
Narjes Jalilvand,
Yousef Baghcheghi,
Masoumeh Fani,
Farimah Beheshti,
Alireza Ebrahimzadeh-Bideskan,
Narges Marefati,
Maryam Moghimian,
Mahmoud Hosseini
Affiliations
Narjes Jalilvand
Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Yousef Baghcheghi
Student Research Committee, Jiroft University of Medical Sciences, Jiroft, Iran
Masoumeh Fani
Department of Anatomy, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
Farimah Beheshti
Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran; Department of Physiology, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Alireza Ebrahimzadeh-Bideskan
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Narges Marefati
Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Maryam Moghimian
Nursing Research Center, Gonabad University of Medical Sciences, Gonabad, Iran; Department of Physiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran; Corresponding author. Department of Physiology, Gonabad University of Medical Sciences, Gonabad, Iran.
Mahmoud Hosseini
Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Corresponding author. Department of Physiology and Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Background: Lipopolysaccharide (LPS) as a particle of Gram-negative bacteria is a main contributer in the pathogenesis of the male reproductive system infectious. Male infertility due to LPS is reported to be related to overproduction reactive oxygen species. This study aimed to investigate the effects of olibanum on oxidative stress and apoptosis in testes and sperm dysfunction induced by LPS. Methods: The male (n = 28) rats were allocated in four groups: control, LPS (1 mg/kg, i.p., 14 days), LPS + Olibanum 100 (100 mg/kg, i.p., 14 days), and LPS + Olibanum 200 (200 mg/kg, i.p., 14 days). Germ cell apoptosis was determined by TUNEL assays and computed using the stereological method. Additionally, semen samples of the animals were analyzed for sperm count and morphology. Oxidative stress indicators were also determined. Results: The count of TUNEL-positive germ cells in LPS-treated rats was more than that in the controls. Treatment of the animals with olibanum significantly attenuated the number of apoptotic cells compared to the LPS group. The sperm count and those with a normal morphology in LPS-treated rats was lower than that in the controls. Administration of olibanum significantly improved the sperms with normal morphology and sperm count. Olibanum treatment also improved superoxide dismutase, catalase, and total thiol in testicular tissue and decreased malondialdehyde. Conclusion: Administering both doses of olibanum in LPS-treated rats had potentially a therapeutic value in reducing germ cell apoptosis, as well as improving sperm parameters.
