Simultaneous Separation and Analysis of Five Compounds in <i>Cibotium barometz</i> by Micellar Electrokinetic Chromatography with Large-Volume Sample Stacking
Lili Wang,
Huifeng Xu,
Lishuang Yu,
Zaishi Zhu,
Hongzhi Ye,
Linglong Liu,
Xihai Li,
Jun Peng
Affiliations
Lili Wang
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Huifeng Xu
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Lishuang Yu
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Zaishi Zhu
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Hongzhi Ye
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Linglong Liu
College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Xihai Li
Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
Jun Peng
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
A large volume sample stacking (LVSS) method in micellar electrokinetic chromatography (MEKC) with diode array detector was developed for the simultaneous separation and analysis of five compounds: protocatechuic acid, protocatechuic aldehyde, caffeic acid, syringetin and vanillin in Cibotium barometz. The electrophoretic separation was performed in a 10 mM sodium dodecyl sulfate (SDS) and 50 mM sodium borax-sodium dihydrogen phosphate system (pH = 8.5) with 10% methanol at a separation voltage of 30 kV after optimizing the typical parameters. The detection limits were from 32 pg to 65 pg, which were around 12–27 times lower than MEKC, and 500 times less than reported methods. Finally, the established method was validated to be applicable for the determination of protocatechuic acid and caffeic acid in Cibotium barometz. This proposed method is expected to facilitate the quality control of Cibotium barometz.
