ACE Inhibitory Peptide from Skin Collagen Hydrolysate of <i>Takifugu bimaculatus</i> as Potential for Protecting HUVECs Injury
Shuilin Cai,
Nan Pan,
Min Xu,
Yongchang Su,
Kun Qiao,
Bei Chen,
Bingde Zheng,
Meitian Xiao,
Zhiyu Liu
Affiliations
Shuilin Cai
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Nan Pan
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Min Xu
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Yongchang Su
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Kun Qiao
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Bei Chen
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Bingde Zheng
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Meitian Xiao
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Zhiyu Liu
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Angiotensin-I-converting enzyme (ACE) is a crucial enzyme or receptor that catalyzes the generation of potent vasopressor angiotensin II (Ang II). ACE inhibitory peptides from fish showed effective ACE inhibitory activity. In this study, we reported an ACE inhibitory peptide from Takifugu bimaculatus (T. bimaculatus), which was obtained by molecular docking with acid-soluble collagen (ASC) hydrolysate of T. bimaculatus. The antihypertensive effects and potential mechanism were conducted using Ang-II-induced human umbilical vein endothelial cells (HUVECs) as a model. The results showed that FNLRMQ alleviated the viability and facilitated apoptosis of Ang-II-induced HUVECs. Further research suggested that FNLRMQ may protect Ang-II-induced endothelial injury by regulating Nrf2/HO-1 and PI3K/Akt/eNOS signaling pathways. This study, herein, reveals that collagen peptide FNLRMQ could be used as a potential candidate compound for antihypertensive treatment, and could provide scientific evidence for the high-value utilization of marine resources including T. bimaculatus.
