Comparative Evaluation of <i>Piper nigrum</i>, <i>Rosmarinus officinalis, Cymbopogon citratus</i> and <i>Juniperus communis</i> L. Essential Oils of Different Origin as Functional Antimicrobials in Foods

Abstract

Essential oils can be used as preservatives in foods because of their ability to inhibit bacteria growth in low concentration, which does not influence on foods’ organoleptic properties and does not generate the resistance mechanisms in cells. The aim of that work was to compare the effectiveness of commercial oils from black pepper (Piper nigrum), rosemary (Rosmarinus officinalis), lemongrass (Cymbopogon citratus) and juniper (Juniperus communis L.) with oils obtained in our laboratory. The typical cultivation method was supported by the flow cytometry to detect the cells of very low physiologic and metabolic activity. Our investigation demonstrated that both types of oils can effectively inhibit the growth of saprophytic bacteria P. orientalis. The oils distilled in our laboratory had a bacteriostatic effect at a lower concentration, which is important for application in the food industry. Flow cytometry analyzes and confirmed the thesis that essential oils do not have a germicidal effect on bacteria cells.

Keywords

• antibacterial mode of action
• bacteriostatic activity
• essential oils
• flow cytometry
• viable but nonculturable cells