Frontiers in Neuroscience (Nov 2018)
Voxel-Based Statistical Analysis of 3D Immunostained Tissue Imaging
- Michel E. Vandenberghe,
- Michel E. Vandenberghe,
- Nicolas Souedet,
- Nicolas Souedet,
- Anne-Sophie Hérard,
- Anne-Sophie Hérard,
- Anne-Marie Ayral,
- Florent Letronne,
- Yaël Balbastre,
- Yaël Balbastre,
- Elmahdi Sadouni,
- Elmahdi Sadouni,
- Philippe Hantraye,
- Philippe Hantraye,
- Marc Dhenain,
- Marc Dhenain,
- Frédérique Frouin,
- Jean-Charles Lambert,
- Thierry Delzescaux,
- Thierry Delzescaux
Affiliations
- Michel E. Vandenberghe
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Michel E. Vandenberghe
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Nicolas Souedet
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Nicolas Souedet
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Anne-Sophie Hérard
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Anne-Sophie Hérard
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Anne-Marie Ayral
- INSERM U1167, Institut Pasteur de LilleUniversité Lille-Nord de France, Lille, France
- Florent Letronne
- INSERM U1167, Institut Pasteur de LilleUniversité Lille-Nord de France, Lille, France
- Yaël Balbastre
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Yaël Balbastre
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Elmahdi Sadouni
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Elmahdi Sadouni
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Philippe Hantraye
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Philippe Hantraye
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Marc Dhenain
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Marc Dhenain
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- Frédérique Frouin
- Laboratoire Imagerie Moléculaire in vivo (IMIV UMR 1023 Inserm/CEA/Université Paris Sud - ERL 9218 CNRS)Orsay, France
- Jean-Charles Lambert
- INSERM U1167, Institut Pasteur de LilleUniversité Lille-Nord de France, Lille, France
- Thierry Delzescaux
- CEA, DRF, Institut François JacobMolecular Imaging Research Center, Fontenay-aux-Roses, France
- Thierry Delzescaux
- Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay UniversityUMR9199, Fontenay-aux-Roses, France
- DOI
- https://doi.org/10.3389/fnins.2018.00754
- Journal volume & issue
-
Vol. 12
Abstract
Recently developed techniques to visualize immunostained tissues in 3D and in large samples have expanded the scope of microscopic investigations at the level of the whole brain. Here, we propose to adapt voxel-based statistical analysis to 3D high-resolution images of the immunostained rodent brain. The proposed approach was first validated with a simulation dataset with known cluster locations. Then, it was applied to characterize the effect of ADAM30, a gene involved in the metabolism of the amyloid precursor protein, in a mouse model of Alzheimer's disease. This work introduces voxel-based analysis of 3D immunostained microscopic brain images and, therefore, opens the door to localized whole-brain exploratory investigation of pathological markers and cellular alterations.
Keywords
