Comparative Analysis of Intestinal Microbiota in Wild, Domesticated, and Cultured <i>Gymnocypris potanini firmispinatus</i>
Baoshan Ma,
Jiaqi Zhang,
Dapeng Li,
Zhipeng Chu,
Jieya Liu,
Jiali Jin,
Liqiao Zhong
Affiliations
Baoshan Ma
National Agricultural Science Observing and Experimental Station of Chongqing, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China
Jiaqi Zhang
National Agricultural Science Observing and Experimental Station of Chongqing, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China
Dapeng Li
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
Zhipeng Chu
National Agricultural Science Observing and Experimental Station of Chongqing, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China
Jieya Liu
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
Jiali Jin
National Agricultural Science Observing and Experimental Station of Chongqing, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China
Liqiao Zhong
National Agricultural Science Observing and Experimental Station of Chongqing, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China
The impact of the living environments on the intestinal microbiota of Gymnocypris potanini firmispinatus was studied by analyzing intestinal microbiota diversity, composition, and potential function among wild, domesticated, and cultured groups. The results showed that the fish living in the wild environment exhibited the highest alpha diversity of intestinal microbiota. Intestinal microbial communities in the three groups clustered by living environment, with domesticated and cultured groups showing higher similarity. The dominant phylum in the wild group was Proteobacteria, whereas Fusobacteria and Proteobacteria were the predominate bacteria in the domesticated and cultured groups. RsaHF231, Actinobacteria, Fusobacteria, Cyanobacteria, and Firmicutes were the five key bacteria differentially expressed among the groups. Functional prediction revealed significant variation in the L-cysteine degradation III pathway (PWY-5329) between wild and domesticated groups, and in the vitamin E biosynthesis pathway (PWY-1422) between wild and cultured groups (p G. p. firmispinatus and for the formulation of specific probiotics and functional feeds.
