Biosensors (Feb 2023)

Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

  • Teliang Zhang,
  • Xueliang Wang,
  • Youjun Zeng,
  • Songfeng Huang,
  • Xiaoqi Dai,
  • Weifu Kong,
  • Qian Liu,
  • Jiajie Chen,
  • Junle Qu,
  • Yonghong Shao

DOI
https://doi.org/10.3390/bios13020261
Journal volume & issue
Vol. 13, no. 2
p. 261

Abstract

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Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.

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